Kırşehir Ahi Evran Üniversitesi Kurumsal Akademik Arşivi
DSpace@Kırşehir, Kırşehir Ahi Evran Üniversitesi tarafından doğrudan ve dolaylı olarak yayınlanan; kitap, makale, tez, bildiri, rapor, araştırma verisi gibi tüm akademik kaynakları uluslararası standartlarda dijital ortamda depolar, Üniversitenin akademik performansını izlemeye aracılık eder, kaynakları uzun süreli saklar ve yayınların etkisimi artırmak için telif haklarına uygun olarak Açık Erişime sunar.
Güncel Gönderiler
Hepatitis C Virus Genotypes in Patients with Hepatitis C İnfection in Kırşehir Province: Evaluation of 9 Year Results
(Refik Saydam National Public Health Agency (RSNPHA), 2025) Avan Mutlu, Tuğba
Objective: Hepatitis C virus (HCV) is one of the most important causes of chronic hepatitis. Genotype determination before HCV treatment is recommended for patient management. The aim of this study is to determine the HCV genotype distribution in HCV RNA positive samples sent to Kırşehir Training and Research Hospital Medical Microbiology Laboratory. Methods: Blood samples of HCV-RNA positive chronic hepatitis C patients sent for HCV genotype determination between July 1, 2014 and July 1, 2023 were included in our study. Demographic data of the patients were obtained from the hospital electronic information system. HCV-RNA levels were investigated with the realtime PCR method Cobas TaqMan (Roche Diagnostics, Germany) device between 2014 and 2022, and with the Montania 4896 (Anatolia Geneworks, Turkey) device in 2022 and 2023. Results: It was determined that 84 (59%) of the samples were genotype 1, 15 (11%) were genotype 2, 21 (15%) were genotype 3 and 19 (13%) were genotype 4. Additionally, mixed genotype was detected in 3 (2%) patients. Of the samples detected as genotype 1, 14 (10%) were found to be genotype 1a, 58 (41%) were genotype 1b and 12 (8%) were genotype 1. While 123 (87%) of the samples consisted of Turkish patients, 19 (13%) of them consisted of foreign patients. Of these 19 patients, 6 (31.5%) had genotype 1, 2 (10.5%) had genotype 2, 7 (37%) had genotype 3, 3 (16%) had genotype 4 and 1 (%5) It was determined that were in mixed genotype. Conclusion: The distribution of HCV genotypes constantly changes with migration and changing living standards. Determination and follow-up of HCV genotypes allowed regular recording of epidemiological data. In this way, it contribute to more systematic patient follow-up and management. In this study, although HCV genotype 1b was seen at the highest rate, similar to studies conducted throughout our country, genotypes other than genotype 1 were also detected in our patients.
Synthesis, Characterization and Cytotoxicity Analyzes of Novel ABA–Type PNIPAM-b-PLLA-b-PNIPAM Amphiphilic Block Copolymer
(Pleiades Publishing, 2025) Mısır, Murat; Azarkan, Serap Yalçın; Bilgin, Ahmet
Abstract: ABA–type amphiphilic poly(N-isopropylacrylamide)-block-poly(L-lactide)-block-poly(N-isopropylacrylamide) (PNIPAM-b-PLLA-b-PNIPAM) was synthesized via combination of ring opening polymerization (ROP) and atom transfer radical polymerization (ATRP) using the novel bifunctional PLLA–based ATRP macroinitiator bearing 1,2-bis[(3-oxapropyl)oxa]benzene core. For this purpose, at first bifunctional diol initiator, o-bis[(3-hydroxypropyl)oxy]benzene (1), was synthesized by the reaction of catechol and 3-chloro-1-propanol in the presence of sodium hydroxide (NaOH) in ethanol. Secondly, PLLA-diol (HO-PLLA-OH) was prepared by Sn(Oct)2 -catalyzed (ROP) of (L-LA) at 120°C using (1) as initiator in toluene. Thirdly, dibromoester end-functionalized PLLA-based ATRP macroinitiator (Br-PLLA-Br) was synthesized by esterification of hydroxyl end groups of PLLA-diol. Finally ABA-type block copolymer, (PNIPAM-b-PLLA-b-PNIPAM), was synthesized by (ATRP) of NIPAM as monomer using PLLA-based ATRP macroinitiator in presence of copper(I) chloride/tris[2-(dimethylamino)ethyl]amine (CuCl/Me6TREN) as catalyst system in DMF/water at 25°C. Characterization of the molecular structures for synthesized novel macroinitiator and the block copolymer were made by spectroscopic (FTIR and 1H NMR) and chromatographic (GPC) methods. In the application phase of this study, the effectiveness of polymers was examined on cancer cells. Cytotoxicity and metastatic effects were evaluated in vitro on MDA-MB-231 cell lines. As a result, novel ABA-Type PNIPAM-b-PLLA-b-PNIPAM amphiphilic block copolymer, which has been successfully developed, characterized and determined to be non-toxic, can be used as a promising drug delivery system in many areas such as tumor treatments.
Evaluation of the Potential Risk Posed by Emerging Yr5-Virulent and Predominant Races of Puccinia striiformis f. sp. tritici on Bread Wheat (Triticum aestivum L.) Varieties Grown in Türkiye
(Multidisciplinary Digital Publishing Institute (MDPI), 2025) Akan, Kadir; Cat, Ahmet; Yurduseven, Medine; Tekin, Yesim Sıla; Yeken, Mehmet Zahit; Tekin, Mehmet
In this study, the reactions of 70 bread wheat varieties released in Türkiye to five prevalent Pst races, including the Yr5-virulent PSTr-27, were evaluated. Reaction tests of wheat varieties to all races revealed PSTr-27 as the most aggressive race, followed by PSTr-31, PSTr-28, PSTr-29, and PSTr-30. Notably, only seven varieties (Kıraç 66, İkizce 96, Dinç, Altındane, Ziyabey 98, Bayraktar 2000, and Shiro) exhibited moderately resistant reactions to PSTr-27, while the remaining varieties were susceptible. The presence of nine important resistance (Yr) genes in these varieties was also screened at the molecular level. Yr5, Yr15, and Yr26 genes were not detected in any of the varieties and Yr10 and YrSP genes were each detected in only one variety, while the other genes were detected in different ratios. Molecular screening showed that 19 varieties with no resistance genes used in this study displayed susceptible reactions; however, ten varieties that did not carry any resistance genes showed resistant reactions to one or more races, suggesting the presence of unknown or novel resistance sources. Furthermore, gene combinations, particularly Yr10 + Yr18, significantly provided resistance to all Pst races studied. These findings highlight that continual monitoring of PSTr-27, and other Pst races is needed, since it can be a serious threat to wheat production in Türkiye and neighboring countries.
Investigation of the Relationship Between Health Literacy and Rational Drug use in University Students: A Cross-Sectional Study
(Lippincott Williams and Wilkins, 2025) Doğan, Murat; Deniz Doğan, Birgül
Health literacy refers to individuals’ ability to use medications safely and effectively. Despite easy access to information, university students often engage in irrational drug use. Understanding the relationship between health literacy and medication practices in this population is essential for promoting safe medication behaviors. This study aimed to assess health literacy among university students and its association with rational drug use, considering sociodemographic variables. A cross-sectional study was conducted with 320 first-year students at Kirşehir Ahi Evran University. Health literacy was measured using the Turkish Health Literacy Scale-32 (THLS-32), and rational drug use was assessed with the rational drug use Scale (RDUS). Descriptive statistics and nonparametric tests were used. Significance was set at P < .05. The median age was 20, and 74.1% were female. Most participants were single (96.3%) and had social security (80.6%). The median THLS-32 score was 27.0 (interquartile range: 21.0–36.0), and the median RDUS score was 21.0 (interquartile range: 19.0–24.0). A total of 45.6% had inadequate health literacy, and rational drug use levels were generally low. Health literacy was significantly higher among married students (P < .05), and rational drug use was higher among medical students (P < .05). Students from the Black Sea region had significantly higher health literacy than those from other regions (P < .001). There was no significant correlation between THLS-32 and RDUS scores (r = 0.02; P = .771). University students demonstrated low levels of health literacy and rational drug use. Sociodemographic factors such as marital status, region, and academic department influenced these outcomes. Educational interventions are recommended to promote health literacy and safe medication practices.
The Relationship Between Nail Findings and Cardiac Morbidity in Patients Visiting A Dermatology Clinic: A Prospective Study
(Ondokuz Mayis Universitesi, 2025) Bozduman Habip, Fadime; Sivas, Hanife Merve; Metin, Zuhal; Önentaşçı, Nazime Bensu
Recent research highlights that dermatological manifestations, particularly nail disorders, can serve as clinical indicators of underlying cardiovascular pathologie. This study aimed to elucidate the association between specific nail findings and cardiovascular morbidities, including diabetes mellitus, hypertension, hyperlipidemia, coronary artery disease, heart failure, and valvular heart disease. A prospective observational study was conducted involving 103 patients who presented with nail disorders at dermatology clinics within a six-month interval. Data were systematically collected through comprehensive clinical examinations and review of medical records, encompassing demographic and clinical parameters. Logistic regression analysis was utilized to identify independent predictors of cardiovascular outcomes. The study cohort had a mean age of 47 ± 7.2 years, comprising 59% female and 41% male participants. Several nail disorders were identified as significant predictors of cardiovascular pathology. Specifically, the presence of Terry's nails was significantly associated with diabetes mellitus (OR = 5.23; 95% CI: 1.037–26.365, p = 0.045) and coronary artery disease (OR = 6.25; 95% CI: 1.685–23.168, p = 0.006). Muehrcke's lines emerged as significant predictors of heart failure (OR = 5.89; 95% CI: 1.663–20.831, p = 0.006), whereas the identification of red lunula was significantly correlated with valvular heart disease (OR = 4.76; 95% CI: 1.387–16.302, p = 0.013). Distinct nail disorders may serve as valuable clinical markers of underlying cardiovascular diseases. Dermatological assessments could, therefore, contribute to early detection and management of cardiovascular morbidity.
