Kırşehir Ahi Evran Üniversitesi Kurumsal Akademik Arşivi
DSpace@Kırşehir, Kırşehir Ahi Evran Üniversitesi tarafından doğrudan ve dolaylı olarak yayınlanan; kitap, makale, tez, bildiri, rapor, araştırma verisi gibi tüm akademik kaynakları uluslararası standartlarda dijital ortamda depolar, Üniversitenin akademik performansını izlemeye aracılık eder, kaynakları uzun süreli saklar ve yayınların etkisimi artırmak için telif haklarına uygun olarak Açık Erişime sunar.
Güncel Gönderiler
Arachidonoylcyclopropylamide (ACPA) Reduces Viability and İnduces Endoplasmic Reticulum Stress in Glioma Cells
(Refik Saydam National Public Health Agency (RSNPHA), 2025) Karakoç, Elif; Türeli, Merve Ayan; Hanelçi, Rumeysa Havvanur; Ersoy, Ümran; Oskay Halaçli, Sevil
Objective: The endocannabinoid system (ECB) is present throughout the human body and has been extensively studied, particularly in the nervous system. Cannabinoid 1 (CB1) ligands and receptors are primarily found in the central nervous system and its cells. Neuroglioma is an aggressive and lifethreatening cancer that accounts for approximately 50% of primary central nervous system (CNS) tumors. The treatment protocol for gliomas typically includes surgery, radiation therapy or chemotherapy. In this study, we evaluated the antiproliferative effects of Arachidonoylcyclopropylamide (ACPA), a CB1 receptor agonist, on H4 human neuroglioma cell line. Methods: In this study, we investigated the antiproliferative, pro-apoptotic, antimigratory and organelle-stress-related effects of ACPA on H4 neuroglioma cells. xCELLigence cell impedance assay (n=6) was performed on untreated H4 cells exposed to ACPA at half the maximum inhibitory concentration (IC50) dose. Annexin V/PI labeling was performed by flow cytometry on IC50 ACPA-exposed and untreated H4 cells. In addition, immunofluorescence labeling of anti-GRP78, anti-LC3B and anti-beta tubulin antibodies were analyzed to evaluate the effects on organelle-stress relationship (n=3). Results: The IC50 dose (2.1X10-7 M) of ACPA on H4 cells was determined by xCELLigence cell impedance assay and this dose reduced the proliferation capacity of H4 neuroglioma cells in a dose-time dependent manner (p < 0.05). Flow cytometry analysis showed that cells treated with IC50 ACPA had a higher late apoptotic rate (n=3). Immunofluorescence analysis showed a significant increase in GRP78 and LC3B labeling. Furthermore, a significant decrease in beta tubulin immunolabeling was observed compared to the untreated group (p < 0.05). Conclusion: ACPA induced apoptosis, suppressed proliferation and was able to induce endoplasmic reticulum stress and autophagy in neuroglioma cells. These findings highlight the potential of ACPA as a therapeutic agent for neuroglioma refractory to clinical treatment and warrant further functional validation.
Being a Climate Activist in Türkiye: Life Experiences, Psychosocial Challenges, and Emotional Burdens of the Guardians of the Planet
(John Wiley and Sons Inc, 2025) Ediz, Çiçek; Uzun, Sevda
Objective: The study aims to evaluate the life experiences, psychosocial challenges, and emotional burdens of climate activists, the guardians of the planet, related to the climate crisis, using a phenomenological approach. Methods: This research was conducted in November 2023, utilizing an inductive qualitative framework. In-depth individual interviews were undertaken with 21 climate activists living in diverse regions of Türkiye through the WhatsApp mobile application. The research adhered to the Consolidated Criteria for Reporting Qualitative Research (COREQ) checklist. Results: Data analysis, three themes, and seven sub-themes were identified: climate crisis awareness and motives for becoming an activist (experiences, people involved, and personal attributes); the effects of being a climate activist on mental health (emotional burdens and coping with emotional burdens); and actions taken to reduce carbon footprints in addressing the climate crisis (social and behavioral). Conclusion: Our study highlighted the challenges and coping mechanisms encountered by climate activists in their efforts to combat the climate crisis and revealed that these activists undergo psychosocial challenges and emotional burdens as a result of their engagement in this struggle. Certain activists struggle to manage their psychosocial challenges and consequently seek psychological assistance. Psychosocial support programs should be established for activists experiencing distress over climate change.
Hepatitis C Virus Genotypes in Patients with Hepatitis C İnfection in Kırşehir Province: Evaluation of 9 Year Results
(Refik Saydam National Public Health Agency (RSNPHA), 2025) Avan Mutlu, Tuğba
Objective: Hepatitis C virus (HCV) is one of the most important causes of chronic hepatitis. Genotype determination before HCV treatment is recommended for patient management. The aim of this study is to determine the HCV genotype distribution in HCV RNA positive samples sent to Kırşehir Training and Research Hospital Medical Microbiology Laboratory. Methods: Blood samples of HCV-RNA positive chronic hepatitis C patients sent for HCV genotype determination between July 1, 2014 and July 1, 2023 were included in our study. Demographic data of the patients were obtained from the hospital electronic information system. HCV-RNA levels were investigated with the realtime PCR method Cobas TaqMan (Roche Diagnostics, Germany) device between 2014 and 2022, and with the Montania 4896 (Anatolia Geneworks, Turkey) device in 2022 and 2023. Results: It was determined that 84 (59%) of the samples were genotype 1, 15 (11%) were genotype 2, 21 (15%) were genotype 3 and 19 (13%) were genotype 4. Additionally, mixed genotype was detected in 3 (2%) patients. Of the samples detected as genotype 1, 14 (10%) were found to be genotype 1a, 58 (41%) were genotype 1b and 12 (8%) were genotype 1. While 123 (87%) of the samples consisted of Turkish patients, 19 (13%) of them consisted of foreign patients. Of these 19 patients, 6 (31.5%) had genotype 1, 2 (10.5%) had genotype 2, 7 (37%) had genotype 3, 3 (16%) had genotype 4 and 1 (%5) It was determined that were in mixed genotype. Conclusion: The distribution of HCV genotypes constantly changes with migration and changing living standards. Determination and follow-up of HCV genotypes allowed regular recording of epidemiological data. In this way, it contribute to more systematic patient follow-up and management. In this study, although HCV genotype 1b was seen at the highest rate, similar to studies conducted throughout our country, genotypes other than genotype 1 were also detected in our patients.
Synthesis, Characterization and Cytotoxicity Analyzes of Novel ABA–Type PNIPAM-b-PLLA-b-PNIPAM Amphiphilic Block Copolymer
(Pleiades Publishing, 2025) Mısır, Murat; Azarkan, Serap Yalçın; Bilgin, Ahmet
Abstract: ABA–type amphiphilic poly(N-isopropylacrylamide)-block-poly(L-lactide)-block-poly(N-isopropylacrylamide) (PNIPAM-b-PLLA-b-PNIPAM) was synthesized via combination of ring opening polymerization (ROP) and atom transfer radical polymerization (ATRP) using the novel bifunctional PLLA–based ATRP macroinitiator bearing 1,2-bis[(3-oxapropyl)oxa]benzene core. For this purpose, at first bifunctional diol initiator, o-bis[(3-hydroxypropyl)oxy]benzene (1), was synthesized by the reaction of catechol and 3-chloro-1-propanol in the presence of sodium hydroxide (NaOH) in ethanol. Secondly, PLLA-diol (HO-PLLA-OH) was prepared by Sn(Oct)2 -catalyzed (ROP) of (L-LA) at 120°C using (1) as initiator in toluene. Thirdly, dibromoester end-functionalized PLLA-based ATRP macroinitiator (Br-PLLA-Br) was synthesized by esterification of hydroxyl end groups of PLLA-diol. Finally ABA-type block copolymer, (PNIPAM-b-PLLA-b-PNIPAM), was synthesized by (ATRP) of NIPAM as monomer using PLLA-based ATRP macroinitiator in presence of copper(I) chloride/tris[2-(dimethylamino)ethyl]amine (CuCl/Me6TREN) as catalyst system in DMF/water at 25°C. Characterization of the molecular structures for synthesized novel macroinitiator and the block copolymer were made by spectroscopic (FTIR and 1H NMR) and chromatographic (GPC) methods. In the application phase of this study, the effectiveness of polymers was examined on cancer cells. Cytotoxicity and metastatic effects were evaluated in vitro on MDA-MB-231 cell lines. As a result, novel ABA-Type PNIPAM-b-PLLA-b-PNIPAM amphiphilic block copolymer, which has been successfully developed, characterized and determined to be non-toxic, can be used as a promising drug delivery system in many areas such as tumor treatments.
Evaluation of the Potential Risk Posed by Emerging Yr5-Virulent and Predominant Races of Puccinia striiformis f. sp. tritici on Bread Wheat (Triticum aestivum L.) Varieties Grown in Türkiye
(Multidisciplinary Digital Publishing Institute (MDPI), 2025) Akan, Kadir; Cat, Ahmet; Yurduseven, Medine; Tekin, Yesim Sıla; Yeken, Mehmet Zahit; Tekin, Mehmet
In this study, the reactions of 70 bread wheat varieties released in Türkiye to five prevalent Pst races, including the Yr5-virulent PSTr-27, were evaluated. Reaction tests of wheat varieties to all races revealed PSTr-27 as the most aggressive race, followed by PSTr-31, PSTr-28, PSTr-29, and PSTr-30. Notably, only seven varieties (Kıraç 66, İkizce 96, Dinç, Altındane, Ziyabey 98, Bayraktar 2000, and Shiro) exhibited moderately resistant reactions to PSTr-27, while the remaining varieties were susceptible. The presence of nine important resistance (Yr) genes in these varieties was also screened at the molecular level. Yr5, Yr15, and Yr26 genes were not detected in any of the varieties and Yr10 and YrSP genes were each detected in only one variety, while the other genes were detected in different ratios. Molecular screening showed that 19 varieties with no resistance genes used in this study displayed susceptible reactions; however, ten varieties that did not carry any resistance genes showed resistant reactions to one or more races, suggesting the presence of unknown or novel resistance sources. Furthermore, gene combinations, particularly Yr10 + Yr18, significantly provided resistance to all Pst races studied. These findings highlight that continual monitoring of PSTr-27, and other Pst races is needed, since it can be a serious threat to wheat production in Türkiye and neighboring countries.
