Antifungal activity and chemical composition of the essential oil of heracleum platytaenium boiss’s
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This study was performed to identify effects of the essential oil from Heracleum platytaenium Boiss’s plant on the fungi, Fusarium oxysporum f. sp. radicis-lycopersici (FORL) (Sacc.) W.C. Snyder & H. N. Hans., Rhizoctonia solani Kühn, Sclerotinia sclerotiorum (Lib.) de Bary, Botrytis cinerea Pers. Fr and Verticillium dahliae Kleb. Chemical composition of the plant essential oil was identified. According to GC/MS analysis, a total of 12 components were identified, including mainly myristicin (27.47%), octyl acetate (25.10%), 1-octanol (16.90%) and octyl 2-methylbutyrate (9.88%) as basic components. Antifungal activities of the essential oil were determined by using the agar well diffusion method. Each well contained different concentrations [0 (control), 0.5, 1, 1.5, 2, 5 and 10 µl/well-1] of essential oil. As a result, 10 µl of H. platytaenium essential oil inhibited 100% the growth of mycelium of FORL, R. solani and S. sclerotiorum pathogens reduced significantly the growth of mycelium of other pathogens as well. In addition, the concentration-effect tests of the essential oil of H. platytaenium against these plant pathogens were estimated as LC90 obtained in: FORL, (17.53 µl) R. solani, (15.52 µl), S. sclerotiorum (8.83 µl), B. cinerea (15.58 µl) and V. dahliae (149.54 µl). These results could suggest using the essential oil of H. platytaenium plant for controlling some plant pathogens. © 2016 Egyptian Society for Biological Control of Pests. All rights reserved.