A new perspective to aberrations caused by barium and vanadium ions on Lens culinaris Medik

Abstract

This study investigates aberrations caused by barium and vanadium on meristematic cells of Lens culinaris Medik. Barium and vanadium ions at various concentrations (0.05 M, 0.1 M, 0.25 M, 0.5 M, and 1.0 M) were exposed to the seeds of the plant at fixed time interval (12 h). After seedlings, with a microscopic examination images were captured about the root tips. Those images showed that several abnormalities occurred on the plant such as chromosome breakings, chromosome dispersion, bridge chromosome, chromosome adherence, ring chromosome. Variety and number of abnormalities were counted and compared to each other statistically. The results show an increase in abnormalities caused by for both ions with increasing treatment time. Chromosome adherence and chromosome breaking have reverse relationship in which number of occurrence for one of them decreases with increase on other one. Fish bone and chromosome adherence have a positive relationship in which number of one increases with the raise in other's number. Exposed metals have caused formation of ligands with proteins which can prevent the persistence of metal ions in DNA protein cross-links that are involved in DNA formation process.