Antifungal activity and chemical composition of the essential oil of heracleum platytaenium boiss’s

Abstract

This study was performed to identify effects of the essential oil from Heracleum platytaenium Boiss’s plant on the fungi, Fusarium oxysporum f. sp. radicis-lycopersici (FORL) (Sacc.) W.C. Snyder & H. N. Hans., Rhizoctonia solani Kühn, Sclerotinia sclerotiorum (Lib.) de Bary, Botrytis cinerea Pers. Fr and Verticillium dahliae Kleb. Chemical composition of the plant essential oil was identified. According to GC/MS analysis, a total of 12 components were identified, including mainly myristicin (27.47%), octyl acetate (25.10%), 1-octanol (16.90%) and octyl 2-methylbutyrate (9.88%) as basic components. Antifungal activities of the essential oil were determined by using the agar well diffusion method. Each well contained different concentrations [0 (control), 0.5, 1, 1.5, 2, 5 and 10 µl/well-1] of essential oil. As a result, 10 µl of H. platytaenium essential oil inhibited 100% the growth of mycelium of FORL, R. solani and S. sclerotiorum pathogens reduced significantly the growth of mycelium of other pathogens as well. In addition, the concentration-effect tests of the essential oil of H. platytaenium against these plant pathogens were estimated as LC90 obtained in: FORL, (17.53 µl) R. solani, (15.52 µl), S. sclerotiorum (8.83 µl), B. cinerea (15.58 µl) and V. dahliae (149.54 µl). These results could suggest using the essential oil of H. platytaenium plant for controlling some plant pathogens. © 2016 Egyptian Society for Biological Control of Pests. All rights reserved.