Races of Puccinia striiformis f. sp. tritici identified from the coastal areas of Turkey

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is a devastating disease of wheat in Turkey and worldwide. This pathogen can overcome known resistance genes and negatively affect the wheat production. The objectives of this study were to determine the virulence patterns of wheat stripe rust isolates collected from the coastal areas (Aegean and Mediterranean regions) of Turkey, and to examine the genetic diversity of the pathogen populations by simple sequence repeat (SSR) marker analysis. The majority of the isolates collected were virulent at various frequencies on the resistance genes Yr1 (50%), Yr6 (100%), Yr7 (78%), Yr8 (50%), Yr9 (84%), Yr10 (25%), Yr17 (38%), Yr24 (22%), Yr27 (31%), Yr32 (22%), Yr43 (47%), Yr44 (6%), YrSp (41%), YrTr1 (6%), and avirulent on Yr5 and Yr15. Based on the analysis of virulence, all isolates were determined as 25 races and clustered into six virulence groups (VGs). In contrast, the isolates clustered into four molecular groups (MGs) based on genotypic data. All four MGs were found in Aegean region of Turkey, while only three MGs (MG1, MG2 and MG3) were found in the Mediterranean region of the country. Additionally, analysis of molecular variance indicated that 80.9% of genetic variation was found within regions and 19.1% was found between the regions. The results suggest that interregional migration of the pathogen was high. The data can be helpful for the management of stripe rust and understanding the population structure and migration of Pst in Turkey. © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.