The internal bacterial diversity of stored product pests
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Symbiotic bacteria associated with insects play important roles in different physiological processes such as digestion, insect behavior, defense and providing essential nutrition in insect gut. In addition, these bacteria can be used in biocontrol of insect pests using genetic engineering techniques. The first step is to isolate and identify symbiotic bacteria from insects to elucidate their roles, and to use in the development of transgenic strains. For this purpose, we isolated and characterized the bacterial isolates from stored product pests using a combination of conventional tests and 16S rRNA sequence analysis. The bacterial flora of Callosobruchus maculatus included Bacillus pumilus, Staphylococcus sp. and Pantoea sp. Acanthoscelides obtectus flora included Staphylococcus kloosii, Staphylococcus sp., S. saprophyticus and Enterococcus faecalis. The internal flora of Sitotroga cerealella included Staphylococcus succinus, Enterococcus sp. and Staphylococcus sp. Finally, Phthorimaea operculella flora included Bacillus sp., Staphylococcus sciuri, Enterococcus mundtii, E. casseliflavus, Alcaligenes faecalis, Enterobacter sp., Pantoea agglomerans and Pseudomonas fluorescens.