Effects of in ovo injection of lysine and methionine into fertile broiler (parent stock) eggs on hatchability, growth performance, caecum microbiota, and ileum histomorphology
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We investigated the effect of in ovo injection of lysine, methionine, or their mixture into fertile broiler eggs on hatchability, chick weight, growth performance, inner organ development, caecum total aerobic bacteria, E coil, coliforms, Enterobactericaea, and ileal histomorphology of broilers. Three hundred fertile eggs obtained from 60-week-old Ross 308 broiler breeders were used. Before this study, a preliminary study was conducted to determine the optimal amino acid dose. Optimum amino acid dose was determined as 2 mg/0.2 mL. Before replacing hatching machine, eggs were weighed individually and numbered. On day 16 of incubation, these eggs were allocated to treatment groups: negative control (no injection), positive control (distilled water injection 0.2 mL), lysine (2 mg/0.2 mL), methionine (2 mg/0.2 mL), and lysine + methionine (1 + 1 mg/0.2 mL). The hatching window lasted 32 h. During hatching, chicks hatched in the first hour and the last 6 h were discarded from the study to ensure equal hatching time. After hatching, 120 one-day-old healthy chicks were divided into five treatment groups with three replicates, each including eight birds, for 21 days. These chicks were given a starter diet (3080 kcal/kg metabolizable energy and 22% crude protein) during the trial. The results showed that in ovo injection of lysine, methionine, and lysine ; methionine did not affect relative chick weight, livability, growth performance, caecum microbiota, and ileal villi length and thickness. Lysine injection increased hatchability compared with the negative control and methionine-injected groups. Gastrointestinal weight increased in lysine-injected group compared with the negative control group. The in ovo injection of 2 mg/0.2 mL lysine have a positive effect on the hatchability of fertile eggs.