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dc.contributor.authorAlpay Karaoğlu, Şengül
dc.contributor.authorBozdeveci, Arif
dc.contributor.authorPınarbaş Çetin, Müberra
dc.contributor.authorSevim, Elif
dc.contributor.authorSuyabatmaz, Şeyma
dc.identifier.citationAlpay Karaoğlu, Ş., Bozdeveci, A., Pinarbaş Çetin, M., Sevim, E., Suyabatmaz, Ş., & Akpınar, R. (2023). Isolation, characterization, and genetic diversity of Paenibacillus larvae from AFB suspected specimens in the Central and Eastern Black Sea Regions. Biologia, 1-11.en_US
dc.description.abstractAmerican foulbrood is an important bacterial disease affecting the larvae of honey bees (Apis mellifera L.) caused by Paenibacillus larvae. Due to easy transmission of disease and the ability of bacteria to create spores, it is a bacterium resistant to both physical and chemical conditions. The study aims to isolate, perform microbiological analyses, and determine biochemical properties and genotypes P. larvae strains from AFB samples collected from Turkey's Central and Eastern Black Sea regions. An isolation study was conducted on adult bees, larvae, honey, and primary honeycomb samples from suspected colonies in the regions under study. After the purification of bacterial isolates from samples, P. larvae strains were identified using biochemical and molecular methods. The genetic diversity and ERIC types of P. larvae isolates were determined by rep-PCR DNA genotyping using BOX A1R and MBO REP1 primers and multiplex-PCR. A phylogenetic tree of P. larvae strains was constructed in the study. All P. larvae isolates were determined as ERIC I type. According to the rep-PCR results of P. larvae strains, 15 of the 28 isolates were Ab genotype (54%), 7 (25%) Aβ genotype, 4 (14%) AB genotype, 1 (3.5%) αB genotype, and 1 (3.5%) ab genotype. From an epidemiological viewpoint, it was determined that Ab and Aβ genotypes were widely distributed, while other genotypes (AB, αB, and ab) showed less spread. The results of the study will guide researchers in taking relevant measures to prevent and control American foulbrood. © 2023, The Author(s), under exclusive licence to Plant Science and Biodiversity Centre, Slovak Academy of Sciences (SAS), Institute of Zoology, Slovak Academy of Sciences (SAS), Institute of Molecular Biology, Slovak Academy of Sciences (SAS).en_US
dc.publisherSpringer Science and Business Media Deutschland GmbHen_US
dc.subjectApis mellifera Len_US
dc.subjectBlack Sea regionen_US
dc.subjectPaenibacillus larvaeen_US
dc.titleIsolation, characterization, and genetic diversity of Paenibacillus larvae from AFB suspected specimens in the Central and Eastern Black Sea Regionsen_US
dc.contributor.departmentTıp Fakültesien_US
dc.contributor.authorIDElif Sevim / 0000-0002-6455-1333en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US

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