The effect of different extenders on the sperm motility and viability of frozen Turkey semen

Abstract

This study was conducted to develop long-term storage methods for turkey semen using different extenders. During the study, the massage method was used twice a week to collect the semen from five turkeys, a total of 44 times. The collected fresh semen's average ejaculate quantity, sperm concentration, motility and vitality values were determined as 0.22 +/- 0.01 ml, 3.5 +/- 0.17 x10(9) sp/ml, 77.0 +/- 1.44% and 86.2 +/- 0.95 % respectively. In this study, glucose (G) including 5% Dimethyl sulphoxide (DMSO) cryoprotective, tris-glucose (TG), lactated Ringer's (LR), and lactated Ringer's glucose (LRG) extenders were used. The Turkey semen was combined and divided into four equal parts, and subsequently diluted at a ratio of 1:3 and equilibrated at +4(circle)C for 90 minutes. Following equilibration, the samples were frozen in liquid nitrogen vapor to 80(circle)C for five minutes, and stored at -196(circle)C in liquid nitrogen. After freezing and thawing, the highest motility value was obtained from the G extender (43.3%+/- 1.62%) followed by LRG (24.6 +/- 1.53%), LR (12.6 +/- 0.92%) and TG (12.2 +/- 0.66%). The vitality values were recorded as 55.8 +/- 1.89%, 23.8 +/- 1.58%, 21.5 +/- 1.10% and 36.5 +/- 1.59% respectively. The motility and vitality values were significantly (p<0.01) more for the glucose extender than those for'other extenders. Therefore, it was concluded that glucose extender is better option for the long-term storage of Turkey semen.