Effects of some hormones on enzyme activities of carbonic anhydrase from brain in vitro

Abstract

Hormones are 3 classes as steroid hormones, peptide and proteohormones and derived hormones from amino acids. The influence of PTH, E2, T3, T4, TSH and cortizole hormones has been investigated on bovine brain carbonic anhydrase in vitro. The effect of each hormone on carbonic anhydrase was investigated by Wilbur-Andersen method modified by Rickly and coworkers. All of the hormones were determined to have inhibition effect, on bovine brain carbonic anhydrase isoenzyme. The I50 values of hormones caused inhibition were determined by means of activity percentage [I] diagrams. The values were PTH, E2, T3, T4, TSH and cortizole were I50: 1.54 × 10-14 M, 1.72 × 10-11 M, 3.6 × 10-8 M, 4.48 × 10-13 M, 5.73 × 10-9 M, 1.22 × 10-11 M for bovine brain carbonic anhydrase, respectively.