The Effects Of Co-Cultıvatıon In Wheat (Trıtıcum Sp.) And Flax (Lınum Usıtatıssımum L.) On Shoot Regeneratıon Capacıty Of Explants

Abstract

This study was carried out to investigate the relationship between explants from two different genotypes (wheat and flax) cultured together and determine the effects of co-cultivation on tissue culture response of mature embryo explants of wheat (Triticum sp.) and hypocotyl explants of flax (Linum usitatissimum L.). One winter bread wheat (T. aestivum L.) cv. 'Bezostaja 1', one winter durum wheat (Triticum durum Desf.) cv. 'Cakmak 79', and two flax cvs. 'Madaras' and '1886 Sel.' were used in the study. Mature embryos excised from sterilized seeds of wheat cultivars were placed scutellum downwards on a medium containing 3 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) and incubated at 24 +/- 1 degrees C in total darkness for 14 days for callus induction. Hypocotyl segments in 5 mm length were excised from 7-day-old in vitro-grown seedlings of flax. Explants (callus for wheat and hypocotyl for flax) of both genotypes were cultured on MS medium supplemented with 0.20 mg l(-1) 6-benzylaminopurine (BAP) and 0.01 mg naphthalene acetic acid (NAA) for regeneration at 24 +/- 1 degrees C under cool white fluorescent light (27 mu mol m(-2) s(-1)) with a 16-h light/8-h dark photoperiod for 4 weeks. Sixteen explants were cultured in a 4 x 4 matrix in a Petri dish. Eight out of 16 explants for each genotype were cultured together for co-cultivation. 16 explants from each genotype were cultured alone as control. The results showed that co-cultivation between wheat and flax gave rise to the highest scores in comparison with culturing explants alone.