Comparison of The Protective Effects of Betamethasone, Dexamethasone And Methylprednisolone İn İschemia/Reperfusion İnjury of Rat Ovary

Abstract

Our aim is to evaluate the protective effect of dexamethasone, methylprednisolone and betamethasone treatment against ischemia-reperfusion damage created experimentally in rat ovaries. For this study, 30 female Wistar albino rats were used and the rats were separated randomly into five groups consisting of six rats each: Normal ovary, torsion-detorsion, torsion-detorsion + betamethasone 3 mg/kg, torsion-detorsion + dexamethasone 4 mg/kg and torsion-detorsion + methylprednisolone 10 mg/kg. Except for the normal group, an ovarian torsion procedure was implemented in all other groups for three hours. Then, a detorsion procedure was implemented to the groups for three hours. Medications were given intraperitoneally, 30 minutes before the detorsion procedure. Ovaries of all rats were removed and anti-mullerian hormone (AMH) levels were examined. The methylprednisolone treatment seems to be protective for the damage in terms of vascular congestion (p=0.238), inflammation (p=0.575), edema (p=0.118) and cellular degeneration (p=0.523) by preventing the meaningful increase. The dexamethasone and betamethasone treatment seems to be protective for tissue damage in inflammation (p=0.575, 0.299), cellular degeneration (p=0.575, 0.368) and edema (p=0.212, 0.162). For all steroid groups, preantral+antral follicle decrease and atretic follicle increase were prevented. AMH decline was prevented and levels were similar to normal group (methylprednisolone, betamethasone and dexamethasone p values, respectively; 0.872, 0.064, 0.335). In ischemia / reperfusion injury due to ovarian torsion, steroid use reduces damage and protects ovarian reserves. There was no significant difference between dexamethasone, betamethasone and methylprednisolone in terms of success.