Growth Regulators Effects On In Vıtro Shoot Regeneratıon Of Saınfoın (Onobrychıs Satıva Lam.)

Abstract

The effect of different concentrations of BAP (6-benzylaminopurine)-NAA (a-naphthaleneacetic acid) on in vitro propagation from cotyledon node explant of sainfoin (Onobrychis saliva Lam) was studied The developed large numbers of shoots were propagated from explants within 6 weeks on Murashige and Skoog (MS) media The longest shoot length (6 79 cm) was recorded on MS medium containing 1 mg/l BAP 0 02 mg/l NAA The observed difficulties were overcome after pulse treatment with 50 mg/l IBA for 10 min on MS medium for rooting