Neuronal Imaging at 8-Bit Depth to Combine High Spatial and High Temporal Resolution With Acquisition Rates Up To 40 kHz

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John Wiley and Sons Inc

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info:eu-repo/semantics/openAccess

Özet

A challenge in neuroimaging is acquiring frame sequences at high temporal resolution from the largest possible number of pixels. Measuring 1%–10% fluorescence changes normally requires 12-bit or higher bit depth, constraining the frame size allowing imaging in the kHz range. We resolved Ca2+ or membrane potential signals from cell populations or single neurons in brain slices by acquiring fluorescence at 8-bit depth and by binning pixels offline, achieving unprecedented frame sizes at kHz rates. In hippocampal slices stained with the Ca2+ indicator Fluo-4 AM, we resolved transients at 2 kHz from large frames. Along the apical dendrite of a layer-5 pyramidal neuron, we measured Ca2+ signals associated with a back-propagating action potential at 10 kHz. Finally, in the axon initial segment of the same cell type, we recorded an action potential at 40 kHz by voltage-sensitive dye imaging. This approach unlocks the potential for a range of imaging measurements.

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brain slices, calcium imaging, neurons, voltage imaging

Kaynak

Journal of Biophotonics

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18

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3

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Abbas, F., İpek, Ö. Y., Moreau, P., & Canepari, M. (2025). Neuronal Imaging at 8‐Bit Depth to Combine High Spatial and High Temporal Resolution With Acquisition Rates Up To 40 kHz. Journal of Biophotonics, 18(3), e202400513.

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