Permethrin induces histological and cytological changes in the midgut of the predatory bug, Podisus nigrispinus

Abstract

Insecticides used in the agriculture and forestry have side effects on non-target organisms used as natural enemies. This study evaluated the histopathology and cytotoxicity of permethrin on the midgut of the non-target predatory bug, Podisus nigrispinus (Heteroptera: Pentatomidae) used in the biological control of pest insects. The toxicity and survival of this insect were determined using six concentrations of permethrin via ingestion. Histological and ultraestutural changes of the midgut of P. nigrispinus were analyzed after exposure to permethrin. The insecticide caused toxicity in P. nigrispinus with LC50 = 0.46 mu g L-1 and survival of 47% after 72 h of exposure. The histological changes in the midgut were irregularly bordered epithelium, cytoplasmic vacuolization and apocrine secretions in the lumen after 6 h following exposure to the insecticide. Cytotoxic effects such as granules and vacuoles secreted into the lumen, presence of autophagosomes, and dilatation of infolds of the basal plasma membrane were observed in the three regions of the midgut. Cells of the midgut in apoptosis occurred after 12 h of exposure. Permethrin causes toxic effects, inhibits survival, and produces changes in the histology and cytology of the midgut in P. nigrispinus, suggesting that the cell stress induced by this insecticide can disrupt physiological processes such as digestion, compromising the potential of the predator as a biological control agent of pests. The low selectivity of permethrin to a non-target organism such as the predatory bug, P. nigrispinus indicates that the associated use of this insecticide in biological control should be better evaluated. (C) 2018 Elsevier Ltd. All rights reserved.